IL-21 modulates biological characteristics of NK92 cells by upregulating SENP1 expression / 军事医学
Military Medical Sciences
; (12): 419-423,429, 2017.
Article
em Zh
| WPRIM
| ID: wpr-617263
Biblioteca responsável:
WPRO
ABSTRACT
Objective To investigate the roles of SENP1 in regulation of biological characteristics of NK cells.Methods Lentivirus-mediated-Senp1-small-hairpinRNA (shRNA) transduction was applied to NK92 cells.The expression of SENP1 in NK92 cells was determined by real-time PCR and Western blot.The proliferation of NK92 cells was detected by CCK-8 assay.The apoptosis of NK92 cells was determined by Annexin Ⅴ and PI labeling.The cytotoxicity of NK92 cells against K562 cells was evaluated by luciferase reporter assay.Results Treatment of NK92 cells with IL-21 resulted in SENP1 upregulation.Lentivirus mediated SENP1 knockdown reduced proliferation and increased apoptosis in NK-92 cells,but SENP1 inhibition had slight impact on the cytotoxic ability of NK92 cells to kill K562 cells.Conclusion SENP1 mediates the regulatory effect of IL-21 on the proliferation and survival of NK92 cells.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Military Medical Sciences
Ano de publicação:
2017
Tipo de documento:
Article