Construction and Effect of Artificial MicroRNA Targeted on Hepatitis B Virus / 中山大学学报(医学科学版)
Journal of Sun Yat-sen University(Medical Sciences)
; (6): 489-493, 2017.
Article
em Zh
| WPRIM
| ID: wpr-621454
Biblioteca responsável:
WPRO
ABSTRACT
[Objective] To investigate the construction and inhibitory effects of artificial microRNA targeted on hepatitis B virus.[Methods] Four artificial microRNA sequences for HBV gene were designed with Invitrogen online design software (BLOCK-iTTM RNAi Designer),amiRNA eukaryotic expression plasmid were constructed and transfected into human hepatocellular carcinoma cell line HepG2.2.15.Hepatitis B virus DNA in supernatant was detected by quantitative fluorescence PCR.HBsAg and HBeAg detected by ELISA.[Results] All of the four artificial microRNA sequences could down regulate the level of HBV-DNA,HBsAg and HBeAg.miR3 had the most significant effect on inhibiting HBV-DNA replication.[Conclusion] Artificial microRNA sequences can effectively inhibit the replication of HBV in vitro.
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WPRIM
Idioma:
Zh
Revista:
Journal of Sun Yat-sen University(Medical Sciences)
Ano de publicação:
2017
Tipo de documento:
Article