A HPLC Method for Determination of the Content of an analog of Baogongteng A-ATST in the Aqueous Humor of Rabbits / 上海第二医科大学学报
Journal of Shanghai Jiaotong University(Medical Science)
; (6): 20-21, 2001.
Article
em Zh
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ABSTRACT
Objective A HPLC method was established for determination of the content of ATST in the aqueous humor of rabbits. MethodsThe mobile phase was consisting of methanol-1% triethylamine(5743) and omeprazole (OMZ) as internal standard. The detection was carried out with an ultraviolet detector operated at 235nm. ResultsThere was linearity over the range of 2. 056~41.12 ug/ml in the humor aquosus, r=0.9997. The average recovery of ATST was 94.58 %. Intra-day and in- ter-day RSD were less than 5 % and 10 % ( n = 5), respectively. Conclusion The method is reliable. It can be used for the study on the pharmacokinetics of ATST.
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Idioma:
Zh
Revista:
Journal of Shanghai Jiaotong University(Medical Science)
Ano de publicação:
2001
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Article