Correlation between Mitochondrial Membrane Potential and Topiramate′s Neuroprotection / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)2004.
Article
em Zh
| WPRIM
| ID: wpr-638797
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study topiramate′s neuroprotection on primary cultured hippocampal neurons which were injuried by glutamate and its mechanism.Methods The primary cultured hippocampal neurons were made as the research object,and excitotoxicity model was exected with glutamate.Hippocampal neuron survival was assessed by MTT method and hippocampal neuron mitochondrial membrance potential(MMP) was evaluated by fluorescence microscope and flow cytometry.Results Hippocampal neuron survival of normal control was(98.4?0.8)%,and the survival of glutamate model group was(59.6?3.2)%,at the same time,two topiramate′s groups′ cell survivals was(74.1?0.5)% and(79.2?3.4)%,and topiramate with two levels all could obviously increase hippocampal neuron survival((P
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WPRIM
Idioma:
Zh
Revista:
Journal of Applied Clinical Pediatrics
Ano de publicação:
2004
Tipo de documento:
Article