In vivo alternative testing with zebrafish in ecotoxicology
Journal of Veterinary Science
; : 351-357, 2008.
Article
em En
| WPRIM
| ID: wpr-65395
Biblioteca responsável:
WPRO
ABSTRACT
Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhREEGFP), for comparing toxicity levels with the well-known aryl hydrocarbon receptor (AhR)-binding toxicants: 3,3',4,4',5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-p-dioxin, and 3-methylcholanthrene. These toxicants induced a concentration-dependent increase in morphological disruption, indicating toxicity at early life-stages. The transient transgenic zebrafish liver cell line was sensitive enough to these toxicants to express the CYP1A1 regulated enhanced green fluorescent protein. The findings of this study demonstrated that the zebrafish in vivo model might allow for extremely rapid and reproducible toxicological profiling of early life-stage embryo development. We have also shown that the transient transgenic zebrafish liver cell line can be used for research on AhR mechanism studies.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Poluentes Químicos da Água
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Benzo(a)Antracenos
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Peixe-Zebra
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Linhagem Celular
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Bifenilos Policlorados
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Hepatócitos
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Proteínas de Fluorescência Verde
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Dibenzodioxinas Policloradas
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Larva
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Dose Letal Mediana
Limite:
Animals
Idioma:
En
Revista:
Journal of Veterinary Science
Ano de publicação:
2008
Tipo de documento:
Article