Simultaneous determination of four constituents in Shugan Pills by HPLC / 中成药
Chinese Traditional Patent Medicine
; (12): 1850-1854, 2017.
Article
em Zh
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| ID: wpr-658489
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ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Shugan Pills (Toosendan Fructus,Corydalis Rhizoma,Paeoniae Radix Alba,etc.).METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.05% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS Paeoniflorin,naringin,hesperidin and neohesperidin showed good linear relationships within the ranges of 0.040 44-1.011,0.045 20-1.130,0.047 08-1.177 and 0.047 24-1.181 μg (r =0.999 9),whose average recoveries were 100.5%,98.6%,98.4% and 101.3% with the RSDs of 1.5%,0.9%,1.2% and 1.1%,respectively.The contents of various constituents in the samples with three dosage forms from seven manufacturers showed obvious differences.CONCLUSION We should pay attention to the uneven quality of Shugan Pills.
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Idioma:
Zh
Revista:
Chinese Traditional Patent Medicine
Ano de publicação:
2017
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Article