Liquid resuscitation combined with hydrogen inhalation reduce acute kidney injury during septic shock in rats / 中国病理生理杂志

ABSTRACT

AIM:To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2％ hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide (LPS) in rats.METHODS:Male Wistar rats were randomly divided into 4 groups (15 rats per group)control group,septic shock group,septic shock with early fluid resuscitation group (fluid group) and septic shock with early fluid resuscitation plus 2％ hydrogen inhalation group (fluid + H2 group).The rats were ventilated,and a 2％ hydrogen mixture was used in fluid + H2 group.LPS (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in fluid group and fluid + H2 group.RESULTS:Fluid resuscitation with 2％ hydrogen inhalation decreased the levels of serum creatinine,blood urea nitrogen and neutrophil gelatinase-associated lipocalin.It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone.CONCLUSION:Early fluid resuscitation plus 2％ hydrogen inhalation provided more protection against acute kidney injury during septic shock.