Expression of HIF-1αin tissues of gastric cancer and molecular mechanism / 中国免疫学杂志

ABSTRACT

Objective:To analyze the expression levels of the HIF-1αin the tissues of gastric cancer and the molecular mechanism in the pathogenesis of the gastric cancer .Methods:A total of 60 patients resided at the Hehuang valley in Qinghai province and diagnosed to gastric cancer were collected to plateau group .And the 60 patients resided at the plains (Shenzhen) and diagnosed to gastric cancer were collected to plains group .The specimens of gastric cancer in two groups were collected .The levels of HIF-1αprotein were detected by the SP method of the immunological histochemistry and compared between two groups .The levels of HIF-1αgene were detected by the RT-PCR and compared between two groups .And the levels of miR-210 were detected by the RT-PCR and compared between two groups .To analyze the relationship between the expression of HIF-1αand clinicopathological features in patients with gastric cancer.Results:The levels of HIF-1αprotein in plateau group were higher than in plains group (P<0.05).The levels of HIF-1αgene in plateau group were higher than in plains group (P<0.05).The levels of miR-210 in plateau group were higher than in plains group(P<0.05).The positive expression of HIF-1αin gastric cancer was related to tumor differentiation (P=0.0441),invasion depth (P=0.0319),lymph node metastasis(P=0.0253) and TNM staging(P=0.0289).Conclusion:The expression levels of the HIF-1αin the tissues of gastric cancer are upregulated under hypoxia environment , and the HIF-1αcan improve the development of the gastric cancer.