Deletion of Salmonella enterica serovar typhimurium sipC gene
Asian Pacific Journal of Tropical Biomedicine
;
(12): 987-991, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-672439
ABSTRACT
Objective:
To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate.Methods:
In this research, 50 upstream and 30 downstream regions of S. typhimurium sipC gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned into pGEM T-easy vector. The construct was confirmed by PCR and restriction digest.Results:
PCR amplified 320, 206 and 835 bp DNA fragments were subcloned into pET-32 vector resulting with a plasmid called pET-32-sipC up-kan-sip C down.Conclusions:
The new plasmid (pET-32-sipC up-kan-sip C down) is useful for genetic engineering and for future manipulation of S. typhimurium sipC gene.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Asian Pacific Journal of Tropical Biomedicine
Ano de publicação:
2015
Tipo de documento:
Artigo
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