Polypeptides based on CTL, T helper and B-epitopes of HBV antigens induce HLA-A2~+ human PBMCs to produce CD8~+ CTL-mediated cytotoxicity / 第三军医大学学报
Journal of Third Military Medical University
; (24)2002.
Article
em Zh
| WPRIM
| ID: wpr-678384
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore how to trigger an HLA Ⅰ restricted CD8 + T cell response to exogenously synthesized peptides in vitro . Methods A new panel of therapeutic peptides based on the immunodominant B and CTL epitopes of HBV PreS 2 region and HBcAg and the tetanus toxoid common T helper epitopes were synthesized by Merrifield solid phase peptide synthesis, and HLA A2 + human PBMCs were used to investigate the immunological properties of the mimetic peptides. Results The results demonstrated that the peptides could trigger vigorous CD8 + HBV specific CTL responses in vitro specifically and effectively. Conclusion The results reveal that T helper plus B epitopes designing with the introduction of short and flexible linker can remarkably improve the immunogenicity of short peptides and hence produce effective CTL responses in vitro .
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WPRIM
Idioma:
Zh
Revista:
Journal of Third Military Medical University
Ano de publicação:
2002
Tipo de documento:
Article