The expression of toll-like receptor 4 in the endotoxin-induced acute hepatic injury / 中华传染病杂志

ABSTRACT

Objective In order to explore the role of Toll like receptors 4(TLR4) in recognizing endotoxin and initiating inflammatory response, the expression of TLR4 of the liver in D galactosamine(D Gal) and endotoxin induced acute hepatic injury was analyzed. Methods The BALB/C mice with acute hepatic injury were induced with the combination of D Gal(900 mg/kg)and lipopolysaccharide (LPS, 10 ?g/kg) by intraperitoneal administration. To evaluate the hepatic injury, serum transaminase (ALT and AST) and plasma TNF ? were determined and the mortality was observed at the various time point following intraperitoneal injection with D Gal/LPS. The level of TLR4 mRNA was measured by semiquantitative RT PCR. Also the protein expression of TLR4 in the liver was detected by immunohistochemistry. The data was analyzed by the SAS software. Results After 4 hours of intraperitoneal injection of D Gal/LPS, the serum transaminase and plasma TNF ? levels were apparently elevated. The treatedmouse began to die at 7 hours. The mortality reached up to 80% at 10 hours. TLR4 mRNA were expressed at low level in normal mice liver, while it was significantly increased and maintained at higher level following intraperitoneal injection with D Gal/LPS (compare to 0 h time point, P