Disruption of hom Gene Encoding for Homoserine Dehydrogenase of Corynebacterium glutamicum / 中国生物工程杂志
China Biotechnology
; (12)2006.
Article
em Zh
| WPRIM
| ID: wpr-685265
Biblioteca responsável:
WPRO
ABSTRACT
The hom gene encoding for homoserine dehydrogenase was amplified from the genomic DNA of Corynebacterium glutamicum ATCC 13032.After the kanamycin-resistant gene(Km)cassette from plasmid pET28a was inserted into the center of hom,the homKm cassette was then electroporated into the competent cell of C.glutamicum ATCC 13032.And kanamycin-resistant clones were obtained.PCR was performed to confirm whether the Km gene was integrated into the hom gene of these clones and the recombinant strains of hom-disrupted were screened out.Fermentation results showed that the lysine yield of the hom-disrupted strain C.g-homKm-8 reached 4.7 g/L,which was 6.7 times that of C.glutamicum ATCC 13032.
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Índice:
WPRIM
Idioma:
Zh
Revista:
China Biotechnology
Ano de publicação:
2006
Tipo de documento:
Article