Effect of Sinomenine on the Healing of Spinal Compression Fracture in Rabbits / 世界科学技术-中医药现代化
World Science and Technology-Modernization of Traditional Chinese Medicine
; (12): 2058-2062, 2017.
Article
em Zh
| WPRIM
| ID: wpr-696145
Biblioteca responsável:
WPRO
ABSTRACT
To study the mechanism of injection of sinomenine (Sinomenine,SIN)into Jiaji point in the treatment of L1-L5 segment Compression of fracture spine (VCF) in rabbits.Methods:
54 rabbits were randomly divided into blank control group,model group and sinomenine group (group SIN)by the random number table.All the 2 groups established L1-L5 segment VCF model except the blank control group.After molding,the SIN group was injected SIN at both sides of the L1-L5 section of the vertebral column,the model group and the blank control group were injected the same volume of physiological saline as comparison.The 3 groups were treated for 4 weeks.Fracture of vertebral bone growth differentiation factor 2 (Growth differentiation,FACTOR2,GDF2),vascular endothelial growth factor (Vascular endothelial,growth factor,VEGF) and the expression of VEGF-mRNA were determined by immunohistochemical staining method;in venous blood,activities of nitric oxide (Nitric oxide NO),super oxide dissuade (Super oxide dims,unties,SOD) and bone protection Su (OPG) were detected by ELISA;immunofluorescence was used to measure two chitosan,image analysis method was used to detect spinal bone density in L1-L5 segment,the ELISA was used to detect inflammatory factor interleukin 1 beta (IL-1 beta),IL-6 beta,beta IL-17 in the spinal disc.Results:
compared with the model group,in the SIN group,VEGF,VEGFmRNA and GDF2 in L1-L5 segment of spine were highly expressed,and the bone density increased (P<0.05);two chitosan in intervertebral disc increased,IL-1beta,IL-6 beta,IL-17 beta,(P< 0.05);NO in venous blood decreased OPG,SOD increased (P<0.05),the differences were statistically significant.Conclusion:
injection of SIN into Jiaji can promote the healing of VCF in L1-L5 segment in rabbits.
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Índice:
WPRIM
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
World Science and Technology-Modernization of Traditional Chinese Medicine
Ano de publicação:
2017
Tipo de documento:
Article