Detection of drug resistance genes and homology of carbapenem-resistant Klebsiella pneumoniae / 中国感染控制杂志

ABSTRACT

Objective To explore the drug-resistant genotypes and homology of carbapenem-resistant Klebsiella pneumoniae (CRKP).Methods 38 strains of clinically isolated CRKB in a hospital between September 2015 and February 2016 were collected,drug resistance genes were detected with polymerase chain reaction (PCR),homology of strains was analyzed with pulsed-field gel electrophoresis (PFGE).Results All 38 strains were from intensive care unit(ICU) and surgical intensive care unit(SICU),accounting for 39.48％ and 34.21 ％ respectively.38 strains all harboured drug resistance genes blaKPC and blaSHV,6 strains harboured blaCTT.PFGE revealed that strains were divided into types A,B,C,and D,type C was predominant(65.78％,25/38).Of type A strains,strains 14,15,and 16 carried drug resistance genes blaKPC 2,blaSHV,and blaCTT-M-15 respectively,these strains were all isolated from SICU patients,strains 14 and 15 were isolated on the same day,strain 16 was isolated on the following week;of type C strains,homology of strain 10,18,25,and 28 was 100％.strian 10 and 18 were isolated from ICU patients,strains 25 and 28 were isolated from patients in division I of neurology ICU(both were transferred from ICU),and both were isolated during patient hospitalization in ICU,the isolation time only differed for one day.Conclusion The main drugresistant genotypes of CRKB in this hospital are mainly blaKPC and blaSHV,there is epidemic of clone strains in this hospital.