Proliferation inhibition effects of curcumin against CD44+colon cancer cells in vitro / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 850-853, 2018.
Article
em Zh
| WPRIM
| ID: wpr-702830
Biblioteca responsável:
WPRO
ABSTRACT
Objective:To study confirm whether curcumin could inhibit the proliferation of CD44+colon cancer cells. In this study. Methods: MTT method was used to test the proliferation inhibition effects of curcumin against colon cancer cells. And Real-time PCR and Fluorescence-activated cell sorting (FACS) were used to determine CD44 expression level in colon cancer cells after treated with curcumin. Then we used Magnetic-activated cell sorting ( MACS) method to separate CD44 positive and negative cells. And we compared the proliferation inhibition effects of curcumin against these two kinds of cells. Results: Curcumin could inhibit the proliferation of HCT116 and HT-29 colon cancer cell lines. The expression level of CD44 could also be down-regulated after the colon cancer cells were treated with curcumin. Conclusion: Curcumin could inhibit the proliferation of CD44+colon cancer cells. Thus it was suggested that crcumin might have the effect to inhibit the proliferation of colon cancer stem cells and might be used as a promising agents to prevent the relapse and metastasis of colon cancer.
Texto completo:
1
Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Immunology
Ano de publicação:
2018
Tipo de documento:
Article