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Determination of Voriconazole in Human Plasma by HPLC / 中国药房
China Pharmacy ; (12): 4499-4502, 2017.
Article em Zh | WPRIM | ID: wpr-704445
Biblioteca responsável: WPRO
ABSTRACT
OBJECTIVE:To establish a method for the concentration determination of voriconazole in human plasma.METHODS:Plasma samples were precipitated with acetonitrile.Using ketoconazole as internal standard,HPLC method was adopted.The determination was performed on Dionex U-3000 Dimonsil C18 column with mobile phase consisted of triethylamine-glacial acetic acid-water mixed solution (1 ∶ 1∶98,V/V/V,pH was about 4.0)-acetonitrile (40∶60,V/V) at the flow rate of 1.0 mL/min.The detection wavelength was set at 255 nm.The column temperature was 40 ℃,and sample size was 20 μL.RESULTS:The linear range of voriconazole was 0.2-20.0 μg/mL.The limits of quantification was 0.2 μg/mL,and the minimum detection limit was 0.03 μg/mL.RSDs of inter-day and intra-day were lower than 10%.The method recoveries were 92.06%-106.26% (RSD<5%,n=5),and extraction recoveries were 75.62 %-90.59 % (RSD < 5 %,n=5).The plasma concentration of voriconazole in 10 children ranged 0.22-4.90 μg/mL (n =10).CONCLUSIONS:The method is simple,rapid,specific and can be used for drug monitoring of voriconazole.
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Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: China Pharmacy Ano de publicação: 2017 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Idioma: Zh Revista: China Pharmacy Ano de publicação: 2017 Tipo de documento: Article