Prevalence of Escherichia coli Carrying pks Islands in Bacteremia Patients
Annals of Laboratory Medicine
; : 271-273, 2018.
Article
em En
| WPRIM
| ID: wpr-714427
Biblioteca responsável:
WPRO
ABSTRACT
Escherichia coli can harbor genomic pks islands that code for a polyketide-peptide genotoxin known as colibactin. E. coli strains carrying pks islands trigger genetic instability. pks islands have been significantly associated with bacteremia. We investigated the molecular epidemiology of bacteremic E. coli isolates and the prevalence of bacteremia-causing E. coli carrying pks islands. A total of 146 E. coli isolates were collected at a tertiary-care hospital from January 2015 to December 2016. The phylogenetic groups were determined by multiplex PCR. All isolates were screened by PCR for sequence type 131 (ST131)-associated single-nucleotide polymorphisms (SNPs) in mdh and gyrB. For detection of pks islands, we performed PCR for the clbB and clbN genes as colibactin system markers. Phylogenetic group B2 was the most common, accounting for 54.1% (N=79) of the isolates, followed by group D with 29.5% (N=43), group A with 11.6% (N=17), and group B1 with 4.8%. Of the group B2 isolates, 40.5% were ST131 strains and 32.9% carried pks islands. Only three ST131 isolates in group B2 carried the clbB and clbN genes, while the other 23 ST131 isolates did not. The pks gene might not be associated with ST131 strains.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Reação em Cadeia da Polimerase
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Prevalência
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Bacteriemia
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Epidemiologia Molecular
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Escherichia
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Escherichia coli
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Reação em Cadeia da Polimerase Multiplex
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Ilhas
Tipo de estudo:
Prevalence_studies
Limite:
Humans
Idioma:
En
Revista:
Annals of Laboratory Medicine
Ano de publicação:
2018
Tipo de documento:
Article