KLK6 Promotes Growth, Migration, and Invasion of Gastric Cancer Cells
Journal of Gastric Cancer
; : 356-367, 2018.
Article
em En
| WPRIM
| ID: wpr-719161
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
Kallikrein (KLK) proteases are hormone-like signaling molecules with critical functions in different cancers. This study investigated the expression of KLK6 in gastric cancer and its potential role in the growth, migration, and invasion of gastric cancer cells. MATERIALS ANDMETHODS:
In this study, we compared protein levels of KLK6, vascular endothelial growth factor (VEGF), and matrix metallopeptidase (MMP) 9 in normal gastric epithelial and gastric cancer cell lines by western blot. Fluorescence-activated cell sorting was employed to sort 2 clones of SGC-7901 cells with distinct KLK6 expression, namely, KLK6-high (KLK6high) and KLK6-low (KLK6low), which were then expanded. Lastly, immunohistochemical analysis was performed to investigate KLK6 expression in gastric cancer patients.RESULTS:
The expression levels of KLK6, VEGF, and MMP 9, were significantly higher in the gastric cancer cell lines SGC-7901, BGC-823, MKN-28, and MGC-803 than in the normal gastric epithelial cell line GES-1. Compared to KLK6low cells, KLK6high cells showed enhanced viability, colony-forming ability, migration, and invasion potential in vitro. Importantly, immunohistochemical analysis of a human gastric cancer tissue cohort revealed that the staining for KLK6, VEGF, and MMP9 was markedly stronger in the cancerous tissues than in the adjacent normal tissues. KLK6 expression also correlated with that of VEGF and MMP9 expression, as well as several key clinicopathological parameters.CONCLUSIONS:
Together, these results suggest an important role for KLK6 in human gastric cancer progression.Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Peptídeo Hidrolases
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Neoplasias Gástricas
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Técnicas In Vitro
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Calicreínas
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Linhagem Celular
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Western Blotting
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Estudos de Coortes
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Células Clonais
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Fator A de Crescimento do Endotélio Vascular
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Células Epiteliais
Tipo de estudo:
Etiology_studies
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Incidence_studies
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Observational_studies
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Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Journal of Gastric Cancer
Ano de publicação:
2018
Tipo de documento:
Article