Anti-inflammatory Effects of Flavonoids on TNBS-induced Colitis of Rats
The Korean Journal of Physiology and Pharmacology
; : 43-50, 2015.
Article
em En
| WPRIM
| ID: wpr-727827
Biblioteca responsável:
WPRO
ABSTRACT
It has been shown that the extracts including eupatilin and quercetin-3-beta-D-glucuronopyranoside had mucoprotective effects on the esophagus and stomach through their antioxidant activities. This study was designed to investigate the anti-inflammatory effect of these flavonoid compounds in an animal model of inflammatory bowel disease induced by 2,4,6-trinitrobenzene sulfonic acid. Experimental colitis was induced by intracolonic administration of 2,4,6-trinitrobenzene sulfonic acid. Extracts including eupatilin or quercetin-3-beta-D-glucuronopyranoside were orally administered to animals 48, 24, and 1 h prior to the induction of colitis and then again 24 h later. The animals were sacrificed 48 h after by 2,4,6-trinitrobenzene sulfonic acid treatment and the macroscopic appearance of the colonic lesions was scored in a blinded manner on a scale of 1 to 10. The inflammatory response to colitis induction was assessed by measuring myeloperoxidase activity, nitric oxide production, tumor necrosis factor-alpha expression, total glutathione levels, and malondialdehyde concentrations in the colon. The results indicated that extracts including eupatilin and extracts including quercetin-3-beta-D-glucuronopyranoside dose-dependently improved the morphology of the lesions induced by 2,4,6-trinitrobenzene sulfonic acid and reduced the ulcer index accordingly. In addition, rats receiving extracts including eupatilin and extracts including quercetin-3-beta-D-glucuronopyranoside showed significantly decreased levels of mucosal myeloperoxidase activity, nitric oxide production, tumor necrosis factor-alpha expression, and malondialdehyde levels, and increased total glutathione levels. Extracts including eupatilin and extracts including quercetin-3-beta-D-glucuronopyranoside ameliorated the inflammatory response and colonic injury in acute colitis by decreasing oxidative stress and neutrophil activation. Extracts including eupatilin and extracts including quercetin-3-beta-D-glucuronopyranoside may inhibit acute colitis.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Quercetina
/
Estômago
/
Úlcera
/
Flavonoides
/
Doenças Inflamatórias Intestinais
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Fator de Necrose Tumoral alfa
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Espécies Reativas de Oxigênio
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Colite
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Colo
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Peroxidase
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2015
Tipo de documento:
Article