Expression of neurotensin/neuromedin N precursor in murine mast cells
The Korean Journal of Physiology and Pharmacology
; : 495-501, 2001.
Article
em En
| WPRIM
| ID: wpr-728781
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WPRO
ABSTRACT
We have cloned the mouse neurotensin/neuromedin N (NT/N) gene from the murine mast cell line C1.MC/C57.1 for the first time. The murine NT/N cDNA clone consisted of 765 nucleotides and coded for 169 peptide residues with an N-terminal signal peptide, and the C-terminal region contained of one copy of neurotensin (NT) and one copy of neuromedin N (NN). Total of four Lys-Arg dibasic motifs were present; one each at the middle of the open reading frame, at the N-terminal of NN, at the C-terminal of NT, and between NN and NT. Amino acid sequence analysis of the mouse NT/N revealed 90% homology to that of the rat NT/N gene. NT/N is expressed in routine mast cell lines (C1.MC/C57.1 and P815), but not in murine bone marrow-derived mast cells (BMMCs), murine macrophage cell line (RAW 264.7), nor in murine T cell line (EL-4). NT/N mRNA in C1.MC/C57.1 is highly inducible by IgE cross-linking, phorbol myristate acetate, neurotensin, and substance P. Following the treatment of demethylating agent, 5-azacytidine (5-azaC), the NT/N gene was induced in BMMCs in response to IgE cross-linking. 5-azaC-treated BMMCs did not express the NT/N gene without additional stimuli. These findings suggested that the regulation of NT/N gene expression was dependent on the effects of not only gene methylation but also enhancer and/or repressor proteins acting on the NT/N promoter.
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Índice:
WPRIM
Assunto principal:
Proteínas Repressoras
/
Azacitidina
/
Sinais Direcionadores de Proteínas
/
Imunoglobulina E
/
RNA Mensageiro
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Neurotensina
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Acetato de Tetradecanoilforbol
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Substância P
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Expressão Gênica
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Linhagem Celular
Limite:
Animals
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Ano de publicação:
2001
Tipo de documento:
Article