Occurrence of Internal Stipe Necrosis of Cultivated Mushrooms (Agaricus bisporus) Caused by Ewingella americana in Korea
Mycobiology
; : 62-66, 2009.
Article
em En
| WPRIM
| ID: wpr-729207
Biblioteca responsável:
WPRO
ABSTRACT
The internal stipe necrosis of cultivated mushrooms (Agaricus bisporus) is caused by the bacterium Ewingella americana, a species of the Enterobacteriaceae. Recently, Ewingella americana was isolated from cultivated white button mushrooms in Korea evidencing symptoms of internal stipe browning. Its symptoms are visible only at harvest, and appear as a variable browning reaction in the center of the stipes. From these lesions, we isolated one bacterial strain (designated CH4). Inoculation of the bacterial isolate into mushroom sporocarps yielded the characteristic browning symptoms that were distinguishable from those of the bacterial soft rot that is well known to mushroom growers. The results of Gram stain, flagellal staining, and biochemical tests identified these isolates as E. americana. This was verified by pathogenicity, physiological and biochemical characteristics, and the results of an analysis of the 16S rRNA gene sequences and the fatty acids profile. This is the first report of the isolation of E. americana from cultivated white button mushrooms in Korea.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Entorses e Distensões
/
Reação de Maillard
/
Genes de RNAr
/
Agaricales
/
Enterobacteriaceae
/
Ácidos Graxos
/
Coreia (Geográfico)
/
Necrose
Tipo de estudo:
Prognostic_studies
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Mycobiology
Ano de publicação:
2009
Tipo de documento:
Article