Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol-3-acetate Isolated from Calotropis gigantea (Linn.) Flower
Mycobiology
; : 31-36, 2009.
Article
em En
| WPRIM
| ID: wpr-729212
Biblioteca responsável:
WPRO
ABSTRACT
A phytochemical study on the flowe r of Calotropis gigantea (Linn.) using silica gel column chromatography and preparative thin layer chromatography, led to the first time isolation of Di-(2-ethylhexyl) phthalate (compound 1) and anhydrosophoradiol-3-acetate (compound 2). The structures of these compounds were confirmed by spectroscopic analyses (IR, HRTOFMS and NMR). The antibacterial and antifungal activities of ethyl acetate extract, compound 1 and compound 2 were measured using the disc diffusion method. Ethyl acetate extract and compound 1 presented better results than compound 2. The minimum inhibitory concentrations (MICs) of the extract and compounds were found to be in the range of 16~128 microg/ml. The cytotoxicity (LC50) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 14.61 microg/ml for ethyl acetate, 9.19 microg/ml for compound 1 and 15.55 microg/ml for compound 2.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Ácidos Ftálicos
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Artemia
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Sais
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Testes de Sensibilidade Microbiana
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Cromatografia
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Cromatografia em Camada Fina
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Calotropis
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Flores
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Difusão
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Sílica Gel
Idioma:
En
Revista:
Mycobiology
Ano de publicação:
2009
Tipo de documento:
Article