Development of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae
Mycobiology
; : 74-77, 2010.
Article
em En
| WPRIM
| ID: wpr-729543
Biblioteca responsável:
WPRO
ABSTRACT
To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35degrees C. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25degrees C for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fenolsulfonaftaleína
/
Benzenossulfonatos
/
Carbono
/
Celulose
/
Vermelho Congo
/
Diminazena
/
Concentração de Íons de Hidrogênio
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Mycobiology
Ano de publicação:
2010
Tipo de documento:
Article