Large-scale Isolation, Expansion and Characterization of Human Amniotic Epithelial Cells
International Journal of Stem Cells
; : 87-95, 2018.
Article
em En
| WPRIM
| ID: wpr-739918
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND AND OBJECTIVES:
The human Amniotic epithelial cells (AME) derived from amniotic membrane of placenta have been considered as the potential fetal stem cell source with minimal or no ethical concerns and are important therapeutic tool for anti-fibrotic and regenerative therapies. METHODS ANDRESULTS:
Here, we evaluated the isolation, media screening, scale-up and characterization of AME cells. The isolation, expansion of AMEs were performed by sequential passaging and growth kinetics studies. The AMEs were characterized using immunocytochemistry, immunophenotyping, In-vitro differentiation, and anti-fibrotic assays. The growth kinetics study revealed that the AME cultured in Ultraculture (UC) and DMEM knockout (DMEM-KO) have prominently higher growth rate compared to others. Overall, the AMEs cultured from 5 different media retained basic morphological characteristics and the functional characteristics.CONCLUSIONS:
Our result suggests that the AMEs can be successfully cultured in UC based complete media without losing its epithelial cell characteristics even after passaging for passage 2 (P2). However, a careful and methodical pre-clinical and clinical translation studies need to be conducted to show its safety and efficacy.Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Placenta
/
Imuno-Histoquímica
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Cinética
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Criopreservação
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Programas de Rastreamento
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Imunofenotipagem
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Engenharia Tecidual
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Células Epiteliais
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Células-Tronco Fetais
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Terapia Baseada em Transplante de Células e Tecidos
Tipo de estudo:
Prognostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
International Journal of Stem Cells
Ano de publicação:
2018
Tipo de documento:
Article