Inhibition of growth and proliferation of Hep-2 cells by targeting c-myc gene using small interference RNA technology / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 399-402, 2008.
Article
em Zh
| WPRIM
| ID: wpr-749060
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of small interfering RNA (siRNA) targeting c-myc gene in Hep-2 cells.@*METHOD@#siRNA targeting c-myc mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by MTT, morphology, real time PCR assay.@*RESULT@#1) The MTT result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication; 2) The real time PCR result showed c-myc at mRNA level inhibition ratio at 94% in group S3; 3) The morphology result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication, the cell heteromorphism was diminished.@*CONCLUSION@#siRNA targeting c-myc can remarkably suppress the Hep-2 cell growth and multiplication.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Transfecção
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Divisão Celular
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Genes myc
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Apoptose
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Marcação de Genes
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RNA Interferente Pequeno
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Linhagem Celular Tumoral
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Proliferação de Células
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2008
Tipo de documento:
Article