Neoplasms stem cells play an important role in resistance of laryngeal squamous cancer to chemoradiotherapy / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 400-405, 2014.
Article
em Zh
| WPRIM
| ID: wpr-749377
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To determine an approach enriching cancer stem cells from laryngeal cancer cell line. To investigate whether laryngeal cancer stem cells in chemoradiotherapy have the characteristic of resistance.@*METHOD@#CD133+ cells and CD133- cells was detected and isolated from Hep-2 cell line by fluorescence activated cell sorting technology. The cytotoxicities of cisplatin and radiation were investigated by cell counting kit-8(CCK-8) assay. The apoptosis and cell cycle was analyzed with flow cytometry.@*RESULT@#CD133+ cells accounted for a fraction of (2.43 +/- 0.77)% in Hep-2 cell line. CD133+ cells have a more obvious characteristics of cancer stem cells. Different cisplatin and radiation concentrations of for two cell have inhibition, in a certain concentration range and the dosage dependence. Cisplatin and radiation had synergistic inhibitory effects with CD133- cells on the growth of two cell. Moreover, cell cycle arrest at G0/G1 phase and more apoptosis was induced by synergistic combination. Different concentrations of cetuximab for Hep-2 cells have inhibition, in a certain concentration range and time and the dosage dependence. The half maxial inhibitory concentration (IC50) of cetuximab to Hep-2 cells on 24 h was 1 036.84 microg/L. Cisplatin and radiation had synergistic inhibitory effects with cetuximab on the growth of Hep-2 cell line. Moreover, cell cycle arrest at G0/G1 phase and more apoptosis was induced by synergistic combination.@*CONCLUSION@#Compared with CD133- cells, CD133+ cells subpopulation exhibited extraordinary cancer stem.
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Índice:
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Assunto principal:
Peptídeos
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Farmacologia
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Efeitos da Radiação
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Tolerância a Radiação
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Terapêutica
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Células-Tronco Neoplásicas
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Glicoproteínas
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Antígenos CD
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Ciclo Celular
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Neoplasias Laríngeas
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2014
Tipo de documento:
Article