YB-1 stabilizes HIV-1 genomic RNA and enhances viral production
Protein & Cell
; (12): 591-597, 2013.
Article
em En
| WPRIM
| ID: wpr-757806
Biblioteca responsável:
WPRO
ABSTRACT
HIV-1 utilizes cellular factors for efficient replication. The viral RNA is different from cellular mRNAs in many aspects, and is prone to attacks by cellular RNA quality control systems. To establish effective infection, the virus has evolved multiple mechanisms to protect its RNA. Here, we show that expression of the Y-box binding protein 1 (YB-1) enhanced the production of HIV-1. Downregulation of endogenous YB-1 in producer cells decreased viral production. YB-1 increased viral protein expression by stabilizing HIV-1 RNAs. The stem loop 2 in the HIV-1 RNA packaging signal was mapped to be the YB-1-responsive element. Taken together, these results indicate that YB-1 stabilizes HIV-1 genomic RNA and thereby enhances HIV-1 gene expression and viral production.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Ligação Proteica
/
Replicação Viral
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RNA Viral
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Regulação para Baixo
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Mapeamento Cromossômico
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HIV-1
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Regiões 5' não Traduzidas
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RNA Interferente Pequeno
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Interferência de RNA
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Proteína 1 de Ligação a Y-Box
Limite:
Humans
Idioma:
En
Revista:
Protein & Cell
Ano de publicação:
2013
Tipo de documento:
Article