Construction and application of mCherry red fluorescent protein fusion expression system in lactic acid bacteria / 生物工程学报
Chinese Journal of Biotechnology
; (12): 492-504, 2019.
Article
em Zh
| WPRIM
| ID: wpr-771358
Biblioteca responsável:
WPRO
ABSTRACT
In order to enrich the library of domestic research about new red fluorescent marker in lactic acid bacteria (LAB), we described a new fusion expression system in Lactobacillus plantarum WCFS1 based on the pSIP vector. This system contained red fluorescent protein mCherry as a marker and bile salt hydrolase gene (bsh) as a reporter gene. Moreover, in this study, four different promoters (PsppA, PldhL, P32 and PslpA) were used to regulate the expression of the fusion protein mCherry-BSH, completing the inducible and constitutive expression in lactic acid bacteria. The recombinant protein mCherry-BSH presented activity of red fluorescence and bile salt hydrolase (BSH). The successful construction of the fusion expression system in LAB using a red fluorescent protein mCherry provides favorable conditions for the distribution, intestinal colonization and survival rate of lactic acid bacteria, so as to reveal the function mechanism of its probiotic characteristics; and the system also could lay the foundation for researches on protein expression, cellular localization and properties identification of active protein in lactic acid bacteria.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Probióticos
/
Lactobacillus plantarum
/
Proteínas Luminescentes
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2019
Tipo de documento:
Article