Microbial production of poly (glycolate-co-lactate-co-3-hydroxybutyrate) from glucose and xylose by Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
; (12): 254-262, 2019.
Article
em Zh
| WPRIM
| ID: wpr-771381
Biblioteca responsável:
WPRO
ABSTRACT
Escherichia coli was metabolically engineered to produce poly(glycolate-co-lactate-co-3-hydroxybutyrate) using glucose and xylose as carbon sources. The combinatorial biosynthetic route was constructed by the overexpression of a series of enzymes including D-tagatose 3-epimerase, L-fuculokinase, L-fuculose-phosphate aldolase, aldehyde dehydrogenase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase, and polyhydroxyalkanoate synthase. Overexpression of polyhydroxyalkanoate granule associated protein significantly improved biopolymer synthesis, and the recombinant strain reached 3.73 g/L cell dry weight with 38.72% (W/W) biopolymer content. A co-culture engineering strategy was developed to produce biopolymer from a mixture of glucose and xylose, achieving 4.01 g/L cell dry weight containing 21.54% (W/W) biopolymer. The results of this work offer an approach for simultaneously utilizing glucose and xylose and indicate the potential for future biopolymer production from lignocellulosic biomass.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Poliésteres
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Xilose
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Ácido 3-Hidroxibutírico
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Escherichia coli
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Engenharia Metabólica
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Glucose
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Glicolatos
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Lactatos
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2019
Tipo de documento:
Article