Effects of large tumor suppressor homolog 2 gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 609-612, 2018.
Article
em Zh
| WPRIM
| ID: wpr-772450
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ABSTRACT
OBJECTIVE@#To investigate the effect of large tumor suppressor homolog 2 (LATS2) gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma (OSCC).@*METHODS@#Lentivirous particles were transferred into SCC-25 cell to upregulate LATS2 gene expression. Cell proliferation was detected by CCK-8 assay. Apoptosis was detected through flow cytometry. The expression changes of Bax, Bcl-2, and LATS2 were analyzed by Western blot.@*RESULTS@#Gene transfection increased LATS2 expression. Compared with the control group and pEGFP-control group, SCC-25 cell proliferation in the pGFP-LATS2 group was inhibited, whereas the apoptosis ratio increased (P<0.05). Bcl-2 expression decreased, and Bax expression increased.@*CONCLUSIONS@#Overexpression of LATS2 could inhibit SCC-25 cell proliferation and induce apoptosis.
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Assunto principal:
Fisiologia
/
Neoplasias Bucais
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Carcinoma de Células Escamosas
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Regulação Neoplásica da Expressão Gênica
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Proteínas Serina-Treonina Quinases
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Apoptose
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Proteínas Supressoras de Tumor
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Linhagem Celular Tumoral
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Proliferação de Células
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Genética
Limite:
Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2018
Tipo de documento:
Article