Your browser doesn't support javascript.
loading
An Investigation on the Molecular Characteristics and Intracellular Growth Ability among Environmental and Clinical Isolates of Legionella pneumophila in Sichuan Province, China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 520-530, 2019.
Artigo em Inglês | WPRIM | ID: wpr-773376
ABSTRACT
OBJECTIVE@#To investigate the molecular characteristics and intracellular growth ability of Legionella pneumophila (L. pneumophila) strains from 1989 to 2016 in Sichuan Province, China.@*METHODS@#Seventy-nine isolates of L. pneumophila were collected from environmental and clinical sources, including cooling towers, hot springs, bath water, fountains, and patients, and identified with 16S rRNA gene analysis and serum agglutination assay. The isolates were then typed by Sequence-Based Typing (SBT), and Genotyping of forty-two LP1 strains were analyzed by means of multiple-locus VNTR analysis with 8 loci (MLVA-8). All strains were further analyzed for two virulence genes Legionella vir homologue (lvh) and repeats in structural toxin (rtxA). The intracellular growth ability of 33 selected isolates was determined by examining their interaction with J774 cells.@*RESULTS@#All isolates were identified to L. pneumophila including 11 serogroups, among which the main serogroup were LP1, accounting for 54.43%. Thirty-three different sequence types (STs) from five main clonal groups and five singletons were identified, along with 8 different MLVA patterns. Both the lvh and rtxA loci were found in all 79 strains. Thirty isolates showed high intracellular growth ability in J774 cells.@*CONCLUSION@#L. pneumophila is a potential threat to public health, and effective control and prevention strategies are urgently needed.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas de Bactérias / Toxinas Bacterianas / Microbiologia da Água / RNA Ribossômico 16S / China / Legionella pneumophila / Técnicas de Genotipagem / Genética Tipo de estudo: Estudo prognóstico Limite: Humanos País/Região como assunto: Ásia Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2019 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Proteínas de Bactérias / Toxinas Bacterianas / Microbiologia da Água / RNA Ribossômico 16S / China / Legionella pneumophila / Técnicas de Genotipagem / Genética Tipo de estudo: Estudo prognóstico Limite: Humanos País/Região como assunto: Ásia Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2019 Tipo de documento: Artigo