Effects of TGF-β1 on the expression and secretion of cytokines induced by Aβ in hippocampal neuron and microglia co-cultures / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 385-388 395, 2018.
Article
em Zh
| WPRIM
| ID: wpr-773775
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WPRO
ABSTRACT
OBJECTIVE@#To investigate the neuroprotective effects of transforming growth factor beta 1(TGF-β1) on the expression and secretion of cytokines induced by Aβ in hippocampal neurons and microglial co-cultures.@*METHODS@#Hippocampal neurons and microglia obtained from SD rat were co-cultured. TGF-β1 was applied on day 5 after the neurons and microglia co-cultures were incubated at the concentrations of 5 or 20 ng/ml, Aβ was added 1 h following TGF-β1 application at a concentration of 5 μmol/L. They were incubated for 72 h and then assessed for further studies. Western blot analyses were employed to examine the expression of inducible nitric oxide synthase (iNOS); Real-time PCR and ELISA were used to detect the mRNA expression and secretion of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and insulin-like growth factor-1 (IGF-1).@*RESULTS@#In the hippocampal neuron-microglia co-cultures, Aβ induced upregulation of iNOS, TNF-α and IL-1β, downregulation of IGF-1. TGF-β1 pretreatment ameliorated the pro-inflammatory effects caused by Aβ.@*CONCLUSIONS@#TGF-β1 significantly inhibits the increase in inflammatory cytokines and the decrease in neurotrophic factor which are caused by Aβ-induced microglia activation.
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WPRIM
Assunto principal:
Células Cultivadas
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Citocinas
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Fator de Necrose Tumoral alfa
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Ratos Sprague-Dawley
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Microglia
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Técnicas de Cocultura
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Fator de Crescimento Transformador beta1
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Hipocampo
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Neurônios
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2018
Tipo de documento:
Article