Rapid identification of constituents of Urtica hyperborea using UPLC-ESI-Q-TOF-MS/MS method / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1607-1614, 2019.
Article
em Zh
| WPRIM
| ID: wpr-774515
Biblioteca responsável:
WPRO
ABSTRACT
This paper deals with the application of ultra-performance liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-ESI-Q-TOF-MS/MS) method to rapidly determine and analyze the chemical constituents of methanol extract of Urtica hyperborea. We employed UPLC YMC-Triart C18(2. 1 mm×100 mm,1. 9 μm) column to UPLC analysis with acetonitrile-water(containing 0. 4% formic acid) in gradient as mobile phase. The flow rate was 0. 3 m L·min-1 gradient elution and column temperature was 30℃; the injection volume was 4 μL. ESI ion source was used to ensure the data collected in anegative ion mode. The chemical components of U. hyperborea were identified through retention time,exact relative molecular mass,cleavage fragments of MS/MS and reported data.The results indicated that a total of 31 compounds were identified,including 8 flavonoids,14 phenolic compounds,8 phenylpropanoids(4 coumarins and 4 lignans),and 1 steroidal compound,13 of which were confirmed by comparison. The UPLC-ESI-Q-TOF-MS/MS method could rapid identify the chemical components of U. hyperborea. The above compounds were discovered in U. hyperborea for the first time,which could provide theoretical foundation for further research on the basis of the pharmacodynamics of U. hyperborea.
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Assunto principal:
Fenóis
/
Flavonoides
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Medicamentos de Ervas Chinesas
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Extratos Vegetais
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Química
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Cromatografia Líquida de Alta Pressão
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Lignanas
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Urticaceae
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Espectrometria de Massas em Tandem
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Compostos Fitoquímicos
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2019
Tipo de documento:
Article