Study of heterologous efficient synthesis of β-amyrin and high-density fermentation / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1341-1349, 2019.
Article
em Zh
| WPRIM
| ID: wpr-774550
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WPRO
ABSTRACT
In this study, the synthetic pathway of β-amyrin was constructed in the pre-constructed Saccharomyces cerevisiae chassis strain Y0 by introducing β-amyrin synthase from Glycyrrhiza uralensis, resulting strain Y1-C20-6, which successfully produced β-amyrin up to 5.97 mg·L~(-1). Then, the mevalonate pyrophosphate decarboxylase gene(ERG19), mevalonate kinase gene(ERG12), 3-hydroxy-3-methylglutaryl-CoA synthase gene(ERG13), phosphomevalonate kinase gene(ERG8) and IPP isomerase gene(IDI1)were overexpressed to promoted the metabolic fluxto the direction of β-amyrin synthesis for further improving β-amyrin production, resulting the strain Y2-C2-4 which produced β-amyrin of 10.3 mg·L~(-1)under the shake flask fermentation condition. This is 100% higher than that of strain Y1-C20-6, illustrating the positive effect of the metabolic engineering strategy applied in this study. The titer of β-amyrin was further improved up to 157.4 mg·L~(-1) in the fed-batch fermentation, which was almost 26 fold of that produced by strain Y1-C20-6. This study not only laid the foundation for the biosynthesis of β-amyrin but also provided a favorable chassis strain for elucidation of cytochrome oxidases and glycosyltransferases of β-amyrin-based triterpenoids.
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Assunto principal:
Ácido Oleanólico
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Saccharomyces cerevisiae
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Microbiologia Industrial
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Transferases Intramoleculares
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Glycyrrhiza uralensis
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Fermentação
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Engenharia Metabólica
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Genética
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Metabolismo
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2019
Tipo de documento:
Article