Transcriptomic microarray profiling of peripheral CD4+ T cells from asthmatic patients / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 828-831, 2018.
Article
em Zh
| WPRIM
| ID: wpr-775827
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To identify differentially expressed genes in peripheral blood mononuclear cells between patients with continuous mild-to-moderate asthma and healthy controls using mRNA microarray in order to explore the underlying signaling pathways and clarify the roles of CD4+ T cells in the pathogenesis of asthma.@*METHODS@#Global transcriptomic profiles of the CD4+ T cells were defined by using Agilent Sure Print G3 Human GE 8×60K microarray. Enrichment pathways were analyzed with Ingenuity Pathway Analysis (IPA) software.@*RESULTS@#Compared with controls, 805 genes were up-regulated, 192 were down-regulated in asthma patients. Among these, the expression of 38 annotated genes have varied by 4 times or more. Expression of CD300A was inversely proportional to the absolute value of eosinophils (r=-0.89, P=0.02) as well as the proportion of eosinophils (r=-0.94, P=0.004), while CSF1R was inversely proportional to PD20 (r=-0.83, P=0.04) and AQLQ (r=-0.88, P=0.02) by correlation analysis.@*CONCLUSION@#Numerous pathophysiological pathways may be involved in the pathogenesis of asthma. Above findings have provided a basis for the delineation the pathogenesis of asthma.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Asma
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Leucócitos Mononucleares
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Receptores Imunológicos
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Linfócitos T CD4-Positivos
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Antígenos CD
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Estudos de Casos e Controles
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Receptores de Fator Estimulador das Colônias de Granulócitos e Macrófagos
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Análise de Sequência com Séries de Oligonucleotídeos
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Perfilação da Expressão Gênica
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Biologia Celular
Tipo de estudo:
Observational_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2018
Tipo de documento:
Article