c-Myc-Induced Long Non-Coding RNA Small Nucleolar RNA Host Gene 7 Regulates Glycolysis in Breast Cancer / 한국유방암학회지
Journal of Breast Cancer
; : 533-547, 2019.
Article
em En
| WPRIM
| ID: wpr-783966
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: Recent studies have shown that long non-coding RNA (lncRNA) play an important role in cancer metabolism and development. The lncRNA small nucleolar RNA host gene 7 (SNHG7) was reported to be upregulated in colorectal cancer and contribute to its progression. In the current study, we investigated the role of lncRNA-SNHG7 in breast cancer and explored the underlying mechanism.METHODS:
We monitored the expression of lncRNA-SNHG7 in breast cancer tissues and breast cancer cell lines. We evaluated the effects of lncRNA-SNHG7 on cell proliferation and glycolysis in breast cancer cells by knocking down or overexpressing lncRNA-SNHG7. We searched for the potential microRNA (miRNA) target of lncRNA-SNHG7 and evaluated the effects of the target miRNA on glycolysis. We evaluated the potential regulation of lncRNA-SNHG7 by c-Myc.RESULTS:
LncRNA-SNHG7 was up-regulated in both breast cancer tissues and breast cancer cell lines. Knocking down lncRNA-SNHG7 inhibited breast cancer cell proliferation while overexpressing lncRNA-SNHG7 enhanced cell proliferation. Knocking down lncRNA-SNHG7 resulted in decreased expression of lactate dehydrogenase A (LDHA) and decreased glycolysis. LncRNA-SNHG7 targeted miR-34a-5p to regulate LDHA expression and glycolysis. c-Myc bound to promoter of lncRNA-SNHG7 and positively regulated lncRNA-SNHG7 expression.CONCLUSION:
We demonstrated that c-Myc regulated glycolysis through the lncRNA-SNHG7/miR-34a-5p/LDHA axis in breast cancer cells.Palavras-chave
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Assunto principal:
Mama
/
Neoplasias da Mama
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Neoplasias Colorretais
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Linhagem Celular
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Proteínas Proto-Oncogênicas c-myc
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RNA Nucleolar Pequeno
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MicroRNAs
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Proliferação de Células
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RNA Longo não Codificante
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Glicólise
Idioma:
En
Revista:
Journal of Breast Cancer
Ano de publicação:
2019
Tipo de documento:
Article