The research of separation and purification technology for effective parts f rom Zhizichi decoction by macroporous absorption resina / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 238-241, 2015.
Article
em Zh
| WPRIM
| ID: wpr-790456
Biblioteca responsável:
WPRO
ABSTRACT
Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina . Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry .Tak-ing the content of total iridoid glycosides and total isoflavones as indexes ,the effect of the concentration of sample solution , medicinal herbs on the amount of sample ,concentration and volume of eluent was investigated by single factor test .Results The optimum separation and purification technology was as following :the concentration of sample solution was 0 .1 g/ml ,the volume ratio of resin to material drug was 2∶1 ,the adsorption time was 2 h ,and the sample was firstly eluted with 1 BV wa-ter ,then 6 BV 20% ethanol and 60% ethanol ,and the eluent was collected .Conclusion This optimized separation and purifi-cation technology was reasonable and stable ,and it could be extended to large-scale production applications .
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Pharmaceutical Practice
Ano de publicação:
2015
Tipo de documento:
Article