Application of Cell Culture with Immunocytochemistry and Electron Microscopy to the Diagnosis of Central Neurocytoma
Journal of Korean Neurosurgical Society
; : 1483-1492, 1997.
Article
em Ko
| WPRIM
| ID: wpr-80128
Biblioteca responsável:
WPRO
ABSTRACT
The purpose of this study was to determine the value of primary cell culture in combination with histololgical techniques as a method for the diagnosis of central neurocytoma ; it involved three patients who underwent resection at our hospital and three seen during consultation. Sterile fresh tumor tissues were mechanically and enzymatically dissociated into individual cells and seeded onto poly-L-lysine precoated Aclar coverslips. The cells attached to the surface of the coverslips within 12 to 24 hours, and a delicate cytoplasmic process developed within two to three days of preparation. Immunocytochemical stains for synaptophysin and MAP2 were strongly positive in the cultured tumor cells, and electron microscopic examination also supported their neuronal origin. Cell culture can thus be used as a rapid, reliable and reproducible method for the diagnosis of central neurocytoma, and may be valuable for the diagnosis of small round cell tumors localized in the intra- or periventricular region of the brain.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Encéfalo
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Neoplasias Encefálicas
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Imuno-Histoquímica
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Células Tumorais Cultivadas
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Microscopia Eletrônica
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Sinaptofisina
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Neurocitoma
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Técnicas de Cultura de Células
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Citoplasma
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Diagnóstico
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Ko
Revista:
Journal of Korean Neurosurgical Society
Ano de publicação:
1997
Tipo de documento:
Article