Your browser doesn't support javascript.
loading
The inhibition of inflammatory molecule expression on 3T3-L1 adipocytes by berberine is not mediated by leptin signaling
Article em En | WPRIM | ID: wpr-80366
Biblioteca responsável: WPRO
ABSTRACT
In our previous study, we have shown that berberine has both anti-adipogenic and anti-inflammatory effects on 3T3-L1 adipocytes, and the anti-adipogenic effect is due to the down-regulation of adipogenic enzymes and transcription factors. Here we focused more on anti-inflammatory effect of berberine using real time RT-PCR and found it changes expressions of adipokines. We hypothesized that anti-adipogenicity of berberine mediates anti-inflammtory effect and explored leptin as a candidate mediator of this signaling. We studied this hypothesis by western blot analysis, but our results showed that berberine has no effect on the phosphorylations of STAT-3 and ERK which have important roles on leptin signaling. These results led us to conclude that the anti-inflammatory effect of berberine is not mediated by the inhibition of leptin signal transduction. Moreover, we have found that berberine down-regulates NF-kappaB signaling, one of the inflammation-related signaling pathway, through western blot analysis. Taken together, the anti-inflammatory effect of berberine is not mediated by leptin, and berberine induces anti-inflammatory effect independent of leptin signaling.
Assuntos
Palavras-chave
Texto completo: 1 Índice: WPRIM Assunto principal: Fosforilação / Fatores de Transcrição / Berberina / Transdução de Sinais / Regulação para Baixo / Western Blotting / NF-kappa B / Adipócitos / Leptina / Adipocinas Idioma: En Revista: Nutrition Research and Practice Ano de publicação: 2009 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Fosforilação / Fatores de Transcrição / Berberina / Transdução de Sinais / Regulação para Baixo / Western Blotting / NF-kappa B / Adipócitos / Leptina / Adipocinas Idioma: En Revista: Nutrition Research and Practice Ano de publicação: 2009 Tipo de documento: Article