Genetic characterization analysis of 2B genotype rubella virus strains in Liaoning province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 354-357, 2019.
Article
em Zh
| WPRIM
| ID: wpr-804954
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WPRO
ABSTRACT
Objective@#To analyze the genetic characterization of 2B genotype rubella virus strains first isolated in Liaoning province.@*Methods@#Vero/Slam cells were used to isolate rubella viruses from throat swabs. Fragments of the E1 gene (739 nucleotides) were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and the PCR products were sequenced and analyzed. The phylogenetic trees were constructed with rubella 2B genotypes reference strains.@*Results@#RVi/Liaoning.CHN/13.18/1[2B] was isolateded from the case which had a history of international travel and probably imported from abroad. RVi/Liaoning.CHN/13.18/1[2B] was in the same 1ineage with RVs/Edinburgh.GBR/6.12[2B] and RVs/Kawasaki.JPN/17.11/1[2B], but far from the epidemic strains isolated in China in recent years. RVi/Liaoning.CHN/14.18/1[2B] belonged to the same lineage with rubella virus 2B genotype strains isolated in China from 2014 to 2015, and had the highest homology with RVi/Chouzhou.Anhui.CHN/17.15/2[2B] and RVi/Shaoyang.Zhejiang. CHN/13.13[2B].@*Conclusions@#It was the first time to isolate the 2B genotype rubella virus in Liaoning province and the strain belonged to different 1ineage.
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Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2019
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Article