Structural characterization and identification of major constituents in radix scrophulariae by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 47-54, 2014.
Article
em En
| WPRIM
| ID: wpr-812309
Biblioteca responsável:
WPRO
ABSTRACT
AIM@#To analyze the major constituents in Radix Scrophulariae (Scrophularia ningpoensis).@*METHOD@#Radix Scrophulariae was analyzed by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF MS/MS). Compounds were separated by HPLC using a C18 column and gradient elution of acetonitrile and 0.1 % (V/V) acetic acid-water. Negative ion mode was employed.@*RESULTS@#A total of thirty-six compounds, including fourteen iridoid glycosides, nineteen phenylpropanoid glycosides, and three organic acids, were identified from Radix Scrophulariae based on the accurate mass measurement of precursor and product ions. Twenty-one of the constituents were identified by comparing their retention times (tR) and ESI-MS/MS data with those of reference standards and/or previous publications, while another fifteen compounds were tentatively identified or deduced according to their Q-TOF MS/MS data which afforded sufficient structural information.@*CONCLUSION@#It is believed that this study is useful for the identification of constituents in Radix Scrophulariae, as well as related plants and complex prescriptions.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Medicamentos de Ervas Chinesas
/
Estrutura Molecular
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Raízes de Plantas
/
Espectrometria de Massas por Ionização por Electrospray
/
Scrophularia
/
Espectrometria de Massas em Tandem
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2014
Tipo de documento:
Article