A rapid and sensitive liquid chromatography-tandem mass spectrometric method for determination of actinoside E in rat plasma and application to a pharmacokinetic study / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 427-432, 2013.
Article
em En
| WPRIM
| ID: wpr-812657
Biblioteca responsável:
WPRO
ABSTRACT
A highly sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of actinoside E in rat plasma. The analytes were extracted by ethyl acetate and an analogue of actinoside F was used as the internal standard. The mobile phase consisted of methanol-water (50 50, V/V) containing 0.1% formic acid was delivered at a flow rate of 0.3 mL·min(-1) to a Zorbax SB-C18 column (100 mm × 2.1 mm, 3.5 μm). The detection was performed by electrospray ionization mass spectrometry in the negative multiple reaction monitoring mode with a chromatograph run time of 3.0 min. Calibration curves of actinoside E were linear in the range of 0.5-2 500 ng·mL(-1). In this range, intra- and inter-day precision ranged from 1.7% to 7.5% and 2.0% to 8.9%, respectively. The accuracy ranged from 95.7% to 108.6%, and extraction recovery from 83.2% to 85.5%. This method was successfully applied to a pharmacokinetic study of actinoside E in rats after intravenous (5 mg·kg(-1)) and oral (100 mg·kg(-1)) administration, and the results showed that actinoside E was poorly absorbed with an absolute bioavailability being approximately 0.27%.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Sangue
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Farmacocinética
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Extratos Vegetais
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Química
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Cromatografia Líquida de Alta Pressão
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Sensibilidade e Especificidade
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Ratos Sprague-Dawley
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Actinidia
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Quempferóis
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Espectrometria de Massas em Tandem
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2013
Tipo de documento:
Article