Immune tolerance induced by combined heart-thymus transplantation for heart allograft in rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 47-53, 2007.
Article
em Zh
| WPRIM
| ID: wpr-813938
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To explore the role of combined heart-thymus transplantation for heart allograft in rats.@*METHODS@#Vascularized heart-thymus combined transplantation was performed with microsurgical technique. Graft survival, histopathology, infiltration of CD4+, CD8+ T cells, level and mRNA expressions of IL-2 and IL-4 in the serum and cardiac grafts were investigated.@*RESULTS@#Heart allograft in the controls had a survival time of (6.0+/-0.76) d. heart-thymus combined transplantation in non-thymectomized rats had a survival time of (6.88+/-0.64)d (P<0.05). Heart-thymus combined transplantation in thymectomized rats led to an evident survival time of (14.13+/-5.82)d (P<0.01) for cardiac graft, which further obtained long term survival after short course of treatment with cyclosporine. Pathologic lesion and infiltration of CD4+ and CD8+ T cells in cardiac grafts showed mitigated in the long term survival group. IL-2 level in the serum and cardiac grafts maintained low level in the long term survival group, whereas IL-4 maintained high level.@*CONCLUSION@#Whether thymectomized or not in recipient rats, heart-thymus combined transplantation has a positive effect to protect cardiac graft. Furthermore, in thymectomized rats heart-thymus combined transplantation may lead to evident survival prolongation of the heart grafts, which induces immune tolerance in short course of treatment with cyclosporine.
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1
Índice:
WPRIM
Assunto principal:
Timectomia
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Timo
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Fatores de Tempo
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Transplante
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Transplante Homólogo
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Imunologia de Transplantes
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Sangue
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Linfócitos T CD4-Positivos
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Transplante de Coração
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Interleucina-4
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2007
Tipo de documento:
Article