Proteomic analysis of morphine rabbit myocardium with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 607-611, 2010.
Article
em Zh
| WPRIM
| ID: wpr-814411
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To analyze the morphine rabbit myocardium with matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS).@*METHODS@#Six New Zealand white rabbits were randomly assigned to a control group (Group C) and a morphine group (Group M). Group C were pretreated with bolus injection of saline 1 mL/kg. Group M were pretreated with bolus injection of morphine 3 mg/kg. The myocardium tissue proteins of the rabbits 24 hours after the injection of morphine or saline preconditioned were extracted and separated by two dimensional gel electrophoresis(2-DE), and the images were analyzed and different proteins were found. Some of the different proteins were determined with MALDI-TOF-MS.@*RESULTS@#There were 51 protein spots that displayed quantitative changes in expression (P < 0.05), 15 protein spots were chosen for MS analysis, and 8 proteins were preliminarily identified.They were aldose reductase, zinc finger protein 312, src related tyrosine kinase, carbonic anhydrase 12 precursor, electron transfer flavoprotein beta-subunit, glyceraldehyde-3-phosphate dehydrogenase, tumor necrosis factor ligand superfamily member 11 and transmembrane emp24 domain-containing protein.@*CONCLUSION@#These proteins may be involved in the cardioprotection of morphine preconditioning.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Eletroforese em Gel Bidimensional
/
Traumatismo por Reperfusão Miocárdica
/
Distribuição Aleatória
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Precondicionamento Isquêmico Miocárdico
/
Proteoma
/
Proteômica
/
Metabolismo
/
Métodos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2010
Tipo de documento:
Article