Near-infrared fluorescent zinc-dipicolylamine: a new molecular imaging probe to monitor the efficiency of chemotherapy / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 760-764, 2011.
Article
em Zh
| WPRIM
| ID: wpr-814512
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the feasibility of a novel molecular probe of Zn-DPA-PSS794 to monitor the efficiency of doxorubicin to ovarian cancer and compare with Cy5.5-annexin V.@*METHODS@#Efficiency of doxorubicin to OVCAR-8 cells in vitro was measured by MTT assay and flow cytometry. The in vivo studies were performed on an OVCAR-8 xenograft tumor model. Mice were divided into a control group and a treatment group. Each group was divided into 2 subgroups, DPA and annexin V. In the treatment group, the mice were treated with doxorubicin for 2 doses. All mice were performed optical imaging by Zn-DPA-PSS794 or Cy5.5-annexin V, respectively and then sacrificed. The tumor was separated and stained by HE. The expression of caspase-3 protein was measured by Western blot.@*RESULTS@#The IC50 of doxorubicin to OVCAR-8 was 6 μmol/L. The percentage of apoptosis and dead cells was 35% after doxorubicin treatment. In the optical image, photons accumulated in the tumor either by Zn-DPA-PSS794 or Cy 5.5-annexin V in the treatment group. That was negative in the control group. The fluorescence intensity had significant difference between the 2 groups(P<0.001). The nuclei were big and stained with deep color after the cells were stained with HE. The caspase-3 expression was high in the treatment group, while it was low in the control group.@*CONCLUSION@#Zn-DPA-PSS794 as a probe used by optical imaging can monitor the efficiency of doxorubicin to OVCAR-8 xenograft tumor, which is similar to Cy5.5-annexin V.
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Assunto principal:
Compostos Organometálicos
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Neoplasias Ovarianas
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Patologia
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Farmacologia
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Picolinas
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Carbocianinas
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Doxorrubicina
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Apoptose
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Espectroscopia de Luz Próxima ao Infravermelho
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Linhagem Celular Tumoral
Limite:
Animals
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Female
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Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2011
Tipo de documento:
Article