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Effects of LCL161, a Smac mimetic on the proliferation and apoptosis in hepatocellular carcinoma cells / 中南大学学报(医学版)
Article em Zh | WPRIM | ID: wpr-814946
Biblioteca responsável: WPRO
ABSTRACT
OBJECTIVE@#To investigate the effects of LCL161, a Smac mimetic, on the proliferation and apoptosis in hepatocellular carcinoma cells and the underlying mechanisms. 
@*METHODS@#The effect of LCL161 on the cell viability of HepG2 and SMMC7721 cells was measured by MTT assay. The effect of LCL161 at lower concentrations on the proliferation in hepatocellular carcinoma (HCC) cells was detected by colony formation assay. Apoptosis was assessed by flow cytometry with PI staining. The mitochondrial membrane potential was measured by JC-1 staining. The expression of PARP, p-Akt, cIAP1 and XIAP protein was analyzed by Western blot.
@*RESULTS@#LCL161 displayed notable antiproliferative activity on HCC cells at the concentrations of 1-16 μmol/L (P<0.01), with IC50 values of 4.3 and 4.9 μmol/L for HepG2 and SMMC7721 cells, respectively, after treatment for 48 h. LCL161 at lower concentrations obviously inhibited the colony formation of HCC cells. LCL161 induced significant apoptosis in HCC cells (P<0.01), and resulted in the apoptotic rate at (1.5±0.8)% or (1.8±0.6)% , (15.2±2.8)% or (12.2±2.4)%, (28.7±3.0)% or (22.4±2.7)%, (34.6±2.3)% or (30.2±2.4)% for HepG2 cells or SMMC7721 cells at the concentration of 0, 2, 4 or 8 μmol/L, respectively. The result of JC-1 staining indicated that the mitochondrial membrane potential of HCC cells was reduced by LCL161. In addition, LCL161 promoted the cleavage of PARP, down-regulated the protein expression of p-Akt, and degraded cIAP1.
@*CONCLUSION@#LCL161 possesses significant anti-proliferative activity and pro-apoptotic action in HepG2 and SMMC7721 cells, which might be correlated with reduction in mitochondrial membrane potential, down-regulation of p-Akt and degradation of cIAP1.
Assuntos
Texto completo: 1 Índice: WPRIM Assunto principal: Patologia / Farmacologia / Tiazóis / Regulação para Baixo / Sobrevivência Celular / Apoptose / Carcinoma Hepatocelular / Linhagem Celular Tumoral / Ubiquitina-Proteína Ligases / Proliferação de Células Limite: Humans Idioma: Zh Revista: Journal of Central South University(Medical Sciences) Ano de publicação: 2016 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Patologia / Farmacologia / Tiazóis / Regulação para Baixo / Sobrevivência Celular / Apoptose / Carcinoma Hepatocelular / Linhagem Celular Tumoral / Ubiquitina-Proteína Ligases / Proliferação de Células Limite: Humans Idioma: Zh Revista: Journal of Central South University(Medical Sciences) Ano de publicação: 2016 Tipo de documento: Article