Icaritin promotes maturation and mineralization of mouse osteoblast MC3T3-E1 cells through CXCR4/SDF-1 signal pathway / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 571-577, 2017.
Article
em Zh
| WPRIM
| ID: wpr-819079
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To investigate the effect of icaritin on maturation and mineralization of mouse osteoblast MC3T3-E1 cells and its mechanism. Methods: The cultured MC3T3-E1 cells were divided into blank control group, CXC chemokine receptor type 4 (CXCR4) inhibitor (AMD3100) group, icaritin group, and icaritin plus AMD3100 group. The expression of CXCR4, stromal cell-derived factor 1 (SDF-1) and osteogenesis-related genes and proteins were detected by real-time RT-PCR and Western blotting after drug treatment for 24 h. The alkaline phosphatase (ALP) activity was determined with ALP kit on d3 and d6; calcium nodules were detected by alizarin red staining after drug treatment for 14 d. Results: Real time RT-PCR showed that compared with the blank control group, relative expressions of CXCR4, SDF-1 and osteogenesis-related genes in icaritin group were significantly increased (PPCXCR4 gene was decreased (PPPPPConclusion: Icaritin may promote maturation and mineralization of mouse osteoblast MC3T3-E1 cells through CXCR4/SDF-1 signaling pathway.
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Índice:
WPRIM
Assunto principal:
Osteoblastos
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Farmacologia
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Flavonoides
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Calcificação Fisiológica
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Transdução de Sinais
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Células 3T3
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Regulação da Expressão Gênica no Desenvolvimento
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Receptores CXCR4
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Biologia Celular
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Quimiocina CXCL12
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2017
Tipo de documento:
Article