Effects of arecoline on apoptosis of human periodontal ligament fibroblasts / 口腔疾病防治
Journal of Prevention and Treatment for Stomatological Diseases
; (12): 223-226, 2017.
Article
em Zh
| WPRIM
| ID: wpr-822485
Biblioteca responsável:
WPRO
ABSTRACT
Objective @#To observe the effects of arecoline on the related protein expressions in the apoptosis of cultured human periodontal ligament fibroblasts (hPDLFs), such as p-JNK, p-p53, and Bcl-2.@*Methods @#hPDLFs were isolated from human periodontal ligament tissues and expanded in vitro. hPDLFs were treated with different concentrations of arecoline (0, 20, 40 and 80 μg/mL) for 12 h. The proliferative activities were evaluated by MTT. The expressions of p-JNK, p-p53, and Bcl-2 were determined by Western blot. @*Results@#Arecoline inhibited cell proliferation and induced apoptosis protein. The protein level of Bcl-2 was decreased, while p-JNK and p-p53 were increased (P < 0.05). The protein expressions were in a concentration-dependent manner with arecoline. @*Conclusion @#It demonstrates arecoline might contribute to the apoptosis of hPDLFs, and could destroy periodontal tissues.
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WPRIM
Idioma:
Zh
Revista:
Journal of Prevention and Treatment for Stomatological Diseases
Ano de publicação:
2017
Tipo de documento:
Article