Study on the Effects of Deoxyschizandrin on the Proliferation ,Migration and Invasion of Human Nasopharyngeal Carcinoma Cell HONE- 1 via Met/PI 3K/Akt Signaling Pathway / 中国药房
China Pharmacy
; (12): 2376-2381, 2020.
Article
em Zh
| WPRIM
| ID: wpr-825894
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WPRO
ABSTRACT
OBJECTIVE:To study the effects and potential mechani sm of deoxyschizandrin on the proliferation ,migration and invasion of nasopharyngeal carcinoma cell HONE- 1. METHODS :HONE-1 cell was set as cell model ,while CCK- 8 test,wound healing assay and Transwell chamber test were used to detect the proliferation ,migration and invasion ability changes of HONE- 1 cells after treatment with different concentrations [ 0(blank control ),10,20,40 μmol/L] of deoxyschizandrin. Computer molecular docking was performed to analyze the binding ability between deoxyschizandrin and Met protein. Western blotting assay was used to detect the relative protein expressions of p-Met ,p-PI3K,p-Akt,Bcl-2 and N-cadherin in cells. RESULTS :Compared with blank control ,the proliferation ,migration and invasion ability of cells after treated with 10,20,40 μmol/L deoxyschizandrin were all decreased significantly (P<0.05). Results of molecular docking revealed that deoxyschizandrin could stably bind with the activity pocket of Met protein. Results of Western blotting assay demonstrated that compared with blank control ,10,20,40 μmol/L deoxyschizandrin all decreased the relative protein expressions of p-Met ,p-PI3K,p-Akt,Bcl-2 and N-cadherin in cells significantly(P<0.05). CONCLUSIONS :Deoxyschizandrin can inhibit the proliferation ,migration and invasion of HONE- 1 cell via inhibiting the activation of Met/PI 3K/Akt signaling pathway.
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WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2020
Tipo de documento:
Article