Establishment of Acquired Aplastic Anemia SD Rat Model / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 927-931, 2020.
Article
em Zh
| WPRIM
| ID: wpr-827184
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To establish a stable and rapidly rat model acquired aplastic anemia.@*METHODS@#The SD rats were exposed to Csγ-ray at 3.5 and 4.0 Gy ( 91 cGy/min), and intraperitoneally injected with CTX at 35 mg/( kg·d) and CHL at 45 mg/( kg·d) in d 4, 6 and 8 after irradiation; the WBC, platelet and reticulocyte counts in peripheral blood, the smears and nucleated cells counts of bone marrow were observed.@*RESULTS@#The levels of peripheral blood 3-lineage cells of SD rats treated with Csγ-irradiation combined with cyclophosphamide and chloramphenicol were significantly reduced, among which white blood cells, platelets and reticulocytes decreased rapidly, and the number of bone marrow nucleated cells decreased significantly; bone marrow pathological sections showed severe reduction of hematopoietic cells, and the non-hematopoietic cells such as fat cells increased, and a serve or lightly reduction of bone marrow cells were found.@*CONCLUSION@#The rat model established by Csγ-ray irradiation combined with cyclophosphamide and chloramphenicol meets the clinical characteristics of aplastic anemia, and this study provides a stable rat model for the study of new therapeutic drugs for acquired aplastic anemia.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Medula Óssea
/
Células da Medula Óssea
/
Ratos Sprague-Dawley
/
Ciclofosfamida
/
Anemia Aplástica
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2020
Tipo de documento:
Article